FFA Sciences produces novel assay reagents to measure levels of unbound free fatty acids.
These easy-to-use reagents require very little sample. Measurements can be completed in approximately one minute.
We are also developing new fluorescent probes for the following metabolites: Arachidonic, DHA, Linoleic, Oleic, Palmitic and Stearic acids. These probes have enhanced specificity for individual FFA and other metabolites and provide a unique platform for Metabolomics.
ADIFAB2 is a high affinity version of our original ADIFAB assay and provides greater sensitivity for low concentrations of unbound free fatty acids. ADIFAB2 is recommended for serum or plasma measurements.
FFA Specific Probes reveal enhanced specificity for individual FFA and other metabolites.
Each of our probes is packaged with a storage buffer, a measuring buffer, and a calibrating standard.
I-FABP is recombinant rat intestinal fatty acid binding protein.
FFA Standards are calibrated aqueous solutions of fatty acids complexed with bovine serum albumin.
Custom Assays for your choice of metabolites.
Please read our Limited Use Product License before ordering our products.
White - fatty acid
Green - acrylodan fluorescence with fatty acid bound
Blue - acrylodan fluorescence with no fatty acid bound
Yellow - ribbon representation of rat intestinal fatty acid binding protein (FABP)
This model is based on the crystal structures determined by Sacchettini and his collaborators.
Scientists at FFA Sciences can advise you on how best to utilize our products and methods. They can provide the following services:
Measure FFAu Levels
We can measure FFAu levels in your samples of biological fluids such as blood, lymph, and interstitial fluids. Also, contact us for information about assays currently in development in our metabolomics program. These probes can be used in the determination of specific FFAu (FFAu profiles).
Enable High Throughput Screening for Drugs
We can help you screen for drugs that affect FFA metabolism in cells or enzymes using high throughput formats.
Determine Binding Affinities and Enzyme Activities
We can determine binding affinities for proteins (for example, FABP, PPAR, and albumin) or other macro molecules for your choice of FFA. In addition, we can measure reaction kinetics in enzymes for which FFA are reactants or products.
Develop Assays for Unbound or Free Levels of Metabolites
We will generate assays for your choice of metabolites by screening our libraries of fluorescently labeled mutant FABPs for specific binding to the chosen metabolite.
Provide Fatty Acid Albumin Complexes
We can provide user-defined complexes for use in buffering FFAu levels and for instrument calibration.