The World's only Method for Measuring Unbound Free Fatty Acids

Innovative Products and Services

FFA Sciences produces novel assay reagents to measure levels of unbound free fatty acids.


These easy-to-use reagents require very little sample. Measurements can be completed in approximately one minute.


Our most widely used products are ADIFAB  and ADIFAB2, fluorescent probes that can be used in a broad spectrum of applications.


We are also developing new fluorescent probes for the following metabolites: Arachidonic, DHA, Linoleic, Oleic, Palmitic and Stearic acids. These probes have enhanced specificity for individual FFA and other metabolites and provide a unique platform for Metabolomics.


Our Products

Probes

ADIFAB is a unique, patented, fluorescent assay that enables you to assay the aqueous (unbound) concentration of free fatty acids in as little as one minute.  

ADIFAB2 is a high affinity version of our original ADIFAB assay and provides greater sensitivity for low concentrations of unbound free fatty acids. ADIFAB2 is recommended for serum or plasma measurements.

FFA Specific Probes reveal enhanced specificity for individual FFA and other metabolites. 

Each of our probes is packaged with a storage buffer, a measuring buffer, and a calibrating standard.


Other Products

I-FABP  is recombinant rat intestinal fatty acid binding protein. 

FFA  Standards are calibrated aqueous solutions of fatty acids complexed with bovine serum albumin.  

Custom Assays for your choice of metabolites.


Please read our Limited Use Product License before ordering our products.



Illustration:

White - fatty acid  

Green - acrylodan fluorescence with fatty acid bound

Blue - acrylodan fluorescence with no fatty acid bound 

Yellow - ribbon representation of rat intestinal fatty acid binding protein (FABP) 

This model is based on the crystal structures determined by Sacchettini and his collaborators.

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Our Services

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Scientists at FFA Sciences can advise you on how  best to utilize our products and methods. They can provide the following services:


Measure FFAu Levels  

We can measure FFAu levels in your samples of biological fluids such as blood, lymph, and interstitial fluids.  Also, contact us for information about assays currently in development in our metabolomics program. These probes can be used in the determination of specific FFAu (FFAu profiles).
 

Enable High Throughput Screening for Drugs  

We can help you screen for drugs that affect FFA metabolism in cells or enzymes using high throughput formats.
 

Determine Binding Affinities and Enzyme Activities 

We can determine binding affinities for proteins (for example, FABP, PPAR, and albumin) or other macro molecules for  your choice of FFA. In addition, we can measure reaction kinetics  in enzymes for which FFA are reactants or products.
 

Develop Assays for Unbound or Free Levels of Metabolites  

We will generate assays for your choice of metabolites by screening our libraries of fluorescently labeled mutant FABPs for specific binding to the chosen metabolite.
 

Provide Fatty Acid Albumin Complexes 

We can provide user-defined complexes for use  in buffering FFAu levels and for instrument calibration.