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Metabolomics

Metabolomics is a newly emerging discipline that seeks to determine levels of large numbers (>2000) of metabolites in biological samples. This information is being used for: understanding  physiologic networks at the cellular to whole organism level, defining  states of health and disease, drug development, and nutrient effects  on health and disease.


In metabolomics, most technologies in use and those being developed aim to determine the total amount of each metabolite in a biological sample. Currently, no single  technique is capable of resolving the complete metabolome and therefore  multiple techniques as well as extensive sample preparation are required.


In contrast, FFA Sciences is developing a complementary technology for the determination of the unbound  metabolome. In work supported by the NIH Roadmap Initiative in Metabolomics Technology Development, we are developing individual fluorescent probes for the determination of the concentrations of unbound metabolites in both extra and intracellular fluids.


This technology has the advantage of determining the unbound and therefore biologically active form of  the metabolite, requires virtually no sample preparation, uses a single technology and can provide accurate values for concentrations in real time. 


We are currently focused on developing  probes for unbound FFA and several such FFAu specific probes have been developed. At the same time we are expanding investigations to include other metabolites and would encourage partnerships to develop probes  for specific metabolites.

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FFA Sciences

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