Price Information

ADIFAB Kits include ADIFAB, a storage buffer, a measuring buffer and a standard.

Catalog #: ADI-1-200

Unit Size: 0.2 mg

Price: $350

Catalog #: ADI-1-1000

Unit Size: 1.0 mg

Price: $1100

Prices do not include shipping and taxes. ADIFAB is shipped on blue ice.

For plasma/serum, use ADIFAB2.


ADIFAB



ADIFAB (Acrylodan labeled Intestinal Fatty Acid Binding Protein) is a fluorescent molecule that enables you to assay the aqueous concentration of free fatty acids, also known as unbound free fatty acids.

The assay can be used in a variety of biochemical and clinical applications including the determination of lipase activity, fatty acid binding to membranes and proteins, and serum unbound free fatty acid levels.

Assay Method

You can determine unbound free fatty acid levels in an aqueous solution with a simple, one step procedure that completes in approximately one minute: add a small amount of the ADIFAB reagent to a solution suspected of containing unbound free fatty acid and measure the resulting fluorescence. In the absence of unbound free fatty acids, the ADIFAB probe fluoresces in the blue at 432 nm. In the presence of unbound free fatty acids, the emission shifts to the green with a peak at 505 nm. The ratio of fluorescence at 505 nm and 432 nm allows the concentration of unbound free fatty acids to be determined without further calibration. For further details, refer to the protocols.

Storage

ADIFAB should be stored frozen upon receipt and should be protected from light. After ADIFAB is brought up in a solution, it should be refrigerated. The product is stable in a storage buffer at 4C for >3 months.

Characteristics

  • Accuracy: to greater than 1 nM depending on type of FA
  • Purity : Gel electrophoresis > 95%
  • Form: Lyopholized powder
  • Weight: 15000 Da

Patent Number 5,470,714

Safety

ADIFAB does not contain any hazardous components above 1% or any carcinogens above 0.1% as defined in 29 CFR 1910.1200, the OSHA Hazard Communication Standard.

 

 

Intracellular FFAu levels measured with ADIFAB

 

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